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A Lab-on-a-chip Device for Telomerase Activity Detectionbased on ATP Bioluminescence System

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dc.contributor.author Li, Chengsi en_US
dc.contributor.author Zhan, Wei en_US
dc.contributor.author Xiong, Xingguo en_US
dc.contributor.author Patra, Prabir en_US
dc.date.accessioned 2014-07-16T16:44:52Z
dc.date.available 2014-07-16T16:44:52Z
dc.date.issued 2014-03-28 en_US
dc.identifier.uri https://scholarworks.bridgeport.edu/xmlui/handle/123456789/582
dc.description.abstract Lab-on-a-chip (LOC) devices are a subset of Micro Electro Mechanical Systems (MEMS) devices and also called as Micro Total Analysis Systems (μTAS). Telomerase is an unique enzyme which can keep the length and stability of telomeres in order to protect the chromosomes. Active telomerase can be detected in more than 85% tumor cells. The detection of telomerase activity is very important in cancer diagnoses and the study of telomerase inhibitors. In this poster, we proposed the design of a lab-on-a-chip device which can detect the telomerase activity in a convenient way with a short processing time and micro-amount of sample usage. When the extracted telomerase is mixed with telomerase substrate (TS) primer and the deoxyribonucleotide triphosphate (dNTP) to perform an extension reaction, pyrophosphate (PPi) is released. After the released PPi is converted to ATP, the level of ATP can be detected by the ATP bioluminescence system, which can reflect the activity of telomerase. This LOC device contains one sample inlet, six pre-stored reagents, two mixing zone and two reaction chambers connected by microchannel with diameter of 100μm. COMSOL simulation shows this LOC device works effectively with satisfying results. en_US
dc.language.iso en_US en_US
dc.subject Engineering en_US
dc.subject Faculty research day en_US
dc.subject Bioluminescence system en_US
dc.subject Telomerase en_US
dc.title A Lab-on-a-chip Device for Telomerase Activity Detectionbased on ATP Bioluminescence System en_US
dc.type Presentation en_US
dc.institute.department School of Engineering en_US
dc.institute.name University of Bridgeport en_US
dc.event.location Bridgeport, CT en_US
dc.event.name Faculty Research Day en_US

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