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Enzymatic Characterization of Leishmania major Phosphatidylethanolamine Methyltransferases LmjPEM1 and LmjPEM2

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dc.contributor.author Bibis, Stergios S.
dc.contributor.author Dahlstrom, Kelly
dc.contributor.author Zhu, Tongtong
dc.contributor.author Zufferey, Rachel
dc.date.accessioned 2015-04-16T18:05:01Z
dc.date.available 2015-04-16T18:05:01Z
dc.date.issued 2015-03-27
dc.identifier.uri https://scholarworks.bridgeport.edu/xmlui/handle/123456789/1135
dc.description.abstract Phosphatidylcholine (PC) is the most abundant phospholipid in the membranes of the human parasite Leishmania. It is synthesized via two metabolic routes, the de novo pathway that starts with the uptake of choline, and the threefold methylation of phosphatidylethanolamine. Choline was shown to be dispensable for Leishmania; thus, the methylation pathway likely represents the primary route for PC production. Here, we have identified and characterized two phosphatidylethanolamine methyltransferases, LmjPEM1and LmjPEM2. Both enzymes are expressed in promastigotes as well as in the vertebrate form amastigotes, suggesting that these methyltransferases are important for the development of the parasite throughout its life cycle. Heterologous expression in yeast has demonstrated that LmjPEM1 and LmjPEM2 complement the choline auxotrophy phenotype of a yeast double null mutant lacking phosphatidylethanolamine methyltransferase activity. LmjPEM1 catalyzes the first, and to a lesser extent, the second methylation reaction. In contrast, LmjPEM2 has the capacity to add the second and third methyl group onto phosphatidylethanolamine to yield (lyso)PC; it can also add the first methyl group, albeit with very low efficiency. en_US
dc.language.iso en_US en_US
dc.subject Enzymes en_US
dc.subject Leishmania en_US
dc.subject Phosphatidylcholine (PC) en_US
dc.subject Phosphatidylethanolamine methyltransferases (PEMT) en_US
dc.title Enzymatic Characterization of Leishmania major Phosphatidylethanolamine Methyltransferases LmjPEM1 and LmjPEM2 en_US
dc.type Presentation en_US
dc.institute.department School of Arts and Sciences en_US
dc.institute.name University of Bridgeport en_US
dc.event.location Bridgeport, CT en_US
dc.event.name Faculty Research Day en_US

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