Separation and Characterization of Cells with Varying Magnetic Nanoparticle Concentration
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Authors
Choudhury, Ishita
Patra, Prabir
Petryk, Alicia
Issue Date
2018-03-23
Type
Other
Language
en_US
Keywords
Cells , Magnetic nanoparticles
Alternative Title
Abstract
The activation of magnetic nanoparticles (mNPs) by an alternating magnetic field (AMF) is currently being explored as technique for targeted therapeutic heating of tumors. Engineered nanoparticles have attracted much attention during the last decade because of their size-related properties, large surface-to-volume ratios and high surface reactivity. Parameters such as the size, charge and surface chemistry are examined because they affect the interactions of the nanoparticles with living cells. The mechanism for the uptake and distribution within cells is not fully understood. Optimization of targeted cellular uptake is critical if many nanoparticle-based applications are to become utilized in the clinical and research settings. We propose to develop a method to separate cells based on intracellular mNP concentration which will enable further study and optimization of cellular uptake in tumor cells.