Hydrogel Films of Chitosan and Keratin for Enhanced Wound Healing
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Authors
Upadhyay, Anusha
Chalise, Akshay Sai
Khanal, Babin
Tamang, Bishmita
Khanal, Prashant Bishesh
Shrestha, Swastika
Tiwari, Tribhuwan
Adhikari, Rameshwar
Pokharel, Mishal
Patra, Prabir
Issue Date
2023-03-24
Type
Other
Language
en_US
Keywords
Keratin , Chitosan , Gellan Gum , Wound , SOS-PAGE , MTT assay
Alternative Title
Abstract
Due to different systemic and local factors, the process of wound healing in humans could be delayed which lead to adverse effects such as chronic consequences sometimes resulting in ulcers. Proper measures, both internal and external measures, should be thus undertaken to facilitate and enhance the healing process effectively. Considering the fibroblast recruitment property of keratin and antibacterial property of chitosan, a composite hydrogel film of keratin and chitosan, to be used as wound dressing, has been developed by using the keratin extracted from raw horns of cattle, biomass wastes, and chitosan prepared by deacetylation of commercially available chitin in combination with gellan gum. For the characterization of keratin, FTIR spectroscopy and SOS-PAGE were performed. The prepared film was characterized by employing swelling, permeability, contamination tests, and MTT assay. The SOS-PAGE of extracted keratin showed a band in the range of human keratin. The film showed characteristics of impermeability, swelling capacity which helps wound healing by retaining moisture and absorption of excess exudates. The film showed no growth of any bacterial colony on its surface confirming its ability to resist any further infections. Likewise, the MTT assay showed cell proliferation was well suited on the prepared film.
Description
UB Rise 2023
Department of Biomedical Engineering
Department of Mechanical Engineering
School of Engineering