Ascorbic Acid Determination in Chinese Hamster Ovary Cells Exposed to Nickel and Chromium
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Authors
Jagdeo, Matthew
Issue Date
2022-03-30
Type
Other
Language
en
Keywords
Ascorbic acid , Chinese hamster , Ovary cells , Nickel , Chromium , Epidemiological and in-vitro studies
Alternative Title
Abstract
Epidemiological and in-vitro studies report populations exposed to environmental mixtures of heavy metals have shown increased cancer rates. In order to study this damage,
cytogenetic tests are suitable methods for identifying DNA damage caused by these complex mixtures. Therefore, the chemical interaction is of major concern in the assessment of risk by regulatory agencies. Epidemiological and in-vitro studies reported that Ni(II) and Cr(VI), at relevant environmental levels, have an antagonistic effect in the induction of cytogenetic effects in cell culture. This antagonism can be explained by the hypothesis that Cr(VI) readily crosses the cell membrane and the ability of Ni(II) to form a stable complex with ascorbic acid (ascorbate) and interfere with the normal interconversion between ascorbate(Asc) to DHA, which is an important process for the reduction of Cr(VI) to its active genotoxic form Cr(III). To study the role of ascorbic acid, a reducing agent, in the reduction of Cr(VI) to Cr(III), Chinese Hamster Ovary (CHO) cells were treated with Ni(II) and Cr(VI) alone and in combination and the ascorbate concentration of each treatment was measured.