A Lab-on-a-chip Device for Telomerase Activity Detectionbased on ATP Bioluminescence System
Authors
Li, Chengsi
Zhan, Wei
Xiong, Xingguo
Patra, Prabir
Issue Date
2014-03-28
Type
Presentation
Language
en_US
Keywords
Engineering , Faculty research day , Bioluminescence system , Telomerase
Alternative Title
Abstract
Lab-on-a-chip (LOC) devices are a subset of Micro Electro Mechanical Systems (MEMS) devices and also called as Micro Total Analysis Systems (μTAS). Telomerase is an unique enzyme which can keep the length and stability of telomeres in order to protect the chromosomes. Active telomerase can be detected in more than 85% tumor cells. The detection of telomerase activity is very important in cancer diagnoses and the study of telomerase inhibitors. In this poster, we proposed the design of a lab-on-a-chip device which can detect the telomerase activity in a convenient way with a short processing time and micro-amount of sample usage. When the extracted telomerase is mixed with telomerase substrate (TS) primer and the deoxyribonucleotide triphosphate (dNTP) to perform an extension reaction, pyrophosphate (PPi) is released. After the released PPi is converted to ATP, the level of ATP can be detected by the ATP bioluminescence system, which can reflect the activity of telomerase. This LOC device contains one sample inlet, six pre-stored reagents, two mixing zone and two reaction chambers connected by microchannel with diameter of 100μm. COMSOL simulation shows this LOC device works effectively with satisfying results.