Herpes Simplex Virus Type 2 Infection In Guinea Pigs: Pathogenicity Of Wild-Type And Temperature-Sensitive Mutant Strains And Modifications By These Strains Against Superinfection With Wild-Type Virus
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Authors
Anderson, Christine A.
Issue Date
1981-06-04
Type
Thesis
Language
en_US
Keywords
Guinea pig , Herpes Simplex , Mutation , Pathogenicity
Alternative Title
Abstract
The pathogenicity of herpes simplex virus (HSV) type 2 wild-type strain 186 and four temperature-sensitive (ts) mutants of strain 186 were studied following genital inoculation of female guinea pigs. Infection with WT strain was generally severe with extensive skin lesions in 89% and a mortality rate of 37% in inoculated animals. WT virus was recovered from nerve and non-nerve tissues of all acutely infected animals as well as the majority (71%) of latently infected animals. In marked contrast, afiimals inoculated with ts mutants manifested a remarkably mild infection, with lesions occurring in · only 16% and a mortality rate of 7%. Virus was recovered from only 13% of ts mutant-inoculated animals during acute infection and 7% during latent infection. The extremely low incidence of clinical disease and low rate of recovery from mutant-inoculated animals initiated a study to determine whether prior inoculation with mutant virus could modify the clinical manifestations associated with WT strain virus upon challenge. The degree of protection was determined by signs of clinical infection, virus recovery from nerve and non-nerve tissues during acute and latent phase post-challenge, and complement-required neutralizing antibody titers. Animals which had prior WT virus infection showed 100% resistance to challenge with the homologous strain. Those initially exposed to the mutants showed a variable degree of protection. Identification of WT and ts mutant virus strains was done by biological characterization in selective cell cultures at permissive (33°C) and nonperrnissive (38°C) temperatures. The reported biochemical and genetic characterization of the ts mutants of HSV-2 used in this study in conjunction with the in vivo studies of pathogenicity presented appears to provide a model for understanding the mechanisms of recurrent HSV infection, resistance and latency.
Description
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Citation
C.A. Anderson, "Herpes Simplex Virus Type 2 Infection In Guinea Pigs: Pathogenicity Of Wild-Type And Temperature-Sensitive Mutant Strains And Modifications By These Strains Against Superinfection With Wild-Type Virus", Thesis, School of Arts & Sciences, Univ. of Bridgeport, Bridgeport, CT, 1981.