Fabrication of Electrospun Composite Nanofibers and Their Application in Early Detection of Femtogram C- reactive protein (CRP)

Abstract

The methodology for developing a protein biosensor has been presented and it focuses on the use of carbon nanotubes and polymer nanofibers based nano composites that have been electrospun in the form of “nanowebs”. These nanowebs have been surface modified to function as sensors for detecting C-reactive protein(CRP). Detection sensitivity of CRP was achieved in the femtogram/mL regime from phosphate buffered saline solutions and in human serum. The designed biosensor is three orders more sensitive than ELISA. Sensitivity enhancement was achieved by tuning the charge transduction at the solid /liquid interface on the nanoweb matrix.