Differentiation Of Mesenchymal Stem Cells (Mscs) To Functional Neuron On Graphene-Polycaprolactone Nanoscaffolds
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Authors
Debnath, Debika
Singh, Manisha
Rawat, Sonali
Tiwari, Mahak
Kalluri, Ankarao
Gupta, Deepika
Dharmadhikari, Bhushan
Patra, Prabir
Mohanty, Sujata
Issue Date
2018-03-23
Type
Other
Language
en_US
Keywords
Graphene , Rehabilitation , Stem cells
Alternative Title
Abstract
Spinal cord is an important part of the central nervous system that controls all activities of the body. It is a tubular bundle of nerve fibers and tissues connecting brain to nearly all parts of the body. Nerve cells in an adult human body do not divide and make copies of themselves. Therefore, in case of an injury or damage to any part of spinal cord causes permanent changes to strength, sensation and other body functions. The field of tissue engineering and regenerative medicine which aims to replace and repair damaged tissues, organs or cells entails for effective methods for fabricating biological scaffolds. Here we present synthesis of fibrous scaffolds by a process called electrospinning that can provide a microenvironment in-vitro for differentiation and proliferation of functional neurons from mesenchymal stem cells. These nanofibrous PCL scaffolds with graphene as filler materials are engineered in such a way so as to provide topological, biochemical as well as electrical cues that can enhance neurite extension and penetration. Poly(ε-caprolactone) (PCL) is a FDA approved synthetic biodegradable polyester extensively used in biomedical applications. Graphene, a single layer carbon crystal, based nanomaterials have recently gained considerable interest for tissue engineering applications including osteogenic, neural and differentiation in other lineages due to their favorable chemical, electrical and mechanical properties. Our final aim is that the functional tissues or organs developed in vitro shall be implanted inside body to rehabilitate the biological function that was lost due to injury, abnormality or loss.