Evaluation of Nanosphere Verigene RT-PCR and Microarray Assay Versus Culture in the Detection of Enteric Pathogens
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Authors
Gofstein, Taylor R.
Issue Date
2015-03-27
Type
Presentation
Language
en_US
Keywords
Verigene assay , Nanosphere Verigene , Real-time polymerase chain reaction (PCR)
Alternative Title
Abstract
With gastroenteritis as the second leading cause of morbidity and mortality worldwide (Guerrant et al. 2001), it is imperative to have a laboratory method which is highly rapid, sensitive, and cost-effective to aid in the diagnosis and treatment of affected patients. This validation study seeks to evaluate the Nanosphere Verigene real-time PCR and microarray assay as a potential replacement for the current culture-based methods of testing stool samples for enteric pathogens in the Bridgeport Hospital microbiology lab.