Natural diatoms dopped with AuNPs as drug delivery systems and comparison with diatoms decorated with graphene
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Authors
Chavez, Eva
Briceno, Sarah
Gonzalez, Gema
Issue Date
2022-03-30
Type
Other
Language
en
Keywords
Natural diatoms , AuNPs , Drug delivery systems , Graphene diatoms
Alternative Title
Abstract
The use of natural diatoms is currently a topic of interest for therapeutic applications due to its facilities, low cost, and biocompatibility. Here a comparison between a previous work of diatoms dopped with AuNPs and the possibility of what oculd be adding graphene to the system, or as a replacement of the AuNPs. Here, are reported the chemical modification of diatoms Aulacoseria genus microalgae-derived biosilica from Guayllabamba - Ecuador decorated with gold nanoparticles by In-situ and Ex-situ methods to study the in vitro gentamicin loading and release properties in simulated body fluid (SBF) from the paper “Natural diatoms dopped with AuNPs as drug delivery systems” . Successful decoration of the diatoms and loaded with gentamicin was confirmed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Raman spectroscopy and Fluorescence Microscopy. It follow the In-vitro drug release by using Ultraviolet-Visible Spectroscopy (UV–vis). The results revealed that diatoms decorated with gold nanoparticles using the Ex-situ method (Au/CTAB-Diatom) showed a faster release reaching a maximum of 93% in 10 days and a lower loading rate, while the samples decorated by the In-situ method presented longer and slower release behavior. Fluorescence properties were enhanced after the gentamicin loaded. The advantage of this work is the control of the structural and optical properties of diatoms deco- rated with gold nanoparticles for the gentamicin drug delivery. As for the graphene adhesion is a similar protocol as the decoration with AuNPs, acquiring new features that would be study in next works.